The group originated in the Pokémon anime's first season, where a gang of delinquent Squirtle wore black shades and caused trouble before eventually joining Ash's team. Their leader — permanently stoic behind his tiny sunglasses — quickly became a fan favorite, embodying a kind of effortless, miniature swagger.
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Once Koch established the methods to grow pure cultures of bacteria like tuberculosis and anthrax, he demonstrated for the first time that microbes can cause diseases, a feat that earned him the 1905 Nobel Prize in Physiology or Medicine.